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🧬 SNP Purity Identification
Patent-protected SNP-based purity assay using 24 core loci with Naive Bayes classification for accurate coconut variety identification.
Technology
SNP Panel: 24 loci selected from whole-genome resequencing data:
- High inter-variety polymorphism (Fst > 0.8 across Wenye series vs. CK)
- Zero intra-variety variation within pure lines
- Validated on both KASP and iMLDR platforms
Classifier: Naive Bayes model
P(variety|SNP₁...SNP₂₄) ∝ P(variety) · ∏P(SNPᵢ|variety)- Training set: 20 standard samples per variety
- Validation set: 100+ mixed and field-collected samples
- Accuracy: >99%, purity quantification error ❤️%
Applications
| Scenario | Traditional approach | SNP assay |
|---|---|---|
| Variety authentication | Phenotyping (2−3 years to fruiting) | 24-hour result |
| Seedling purity | Field survey (labor-intensive) | Minimal sample required |
| Hybrid progeny verification | Pedigree tracing (records-dependent) | SNP fingerprint matching |
| Contamination source tracing | No effective method | SNP difference mapping |
Status
- ✅ National Invention Patent granted
- ✅ Applied in batch purity testing for W5 and W6 seedlings
- ✅ Integrated into GPI breeding system (SNP coefficient β=0.35)