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🔬 CT Phenotyping System

Non-destructive X-ray CT phenotyping using a clinical dual-source CT scanner (SOMATOM Definition AS+, Siemens Healthineers). The system covers the complete pipeline from image acquisition to biological interpretation.

Technical Specifications

ParameterValue
ScannerSOMATOM Definition AS+ (dual-source)
Tube voltage120 kV
Tube current200 mAs
Slice thickness0.6 mm
Matrix512 × 512
Reconstruction kernelB30f (medium-smooth)
Total slices7,686 DICOM
Varieties × Stages5 varieties × 5 developmental stages

Pipeline

  1. Automated equatorial slice selection — maximizes cross-sectional area
  2. Tissue segmentation — DeepLabV3+ with improved backbone (Liu et al., 2023, Front. Plant Sci.)
  3. Multi-compartment quantification — fiber, shell, endosperm, water cavity (Lin et al., 2023, PLOS ONE)
  4. 3D volume rendering — non-invasive volumetric analysis (Zhang et al., 2023, Plant Methods)
  5. 78 phenotypic parameters — including CT values, tissue thicknesses, volumes, and morphological indices

Key Publications

  • Zhang Y et al. (2023) Developing non-invasive 3D quantificational imaging for intelligent coconut analysis system with X-ray. Plant Methods 19, 24.
  • Liu Q et al. (2023) An improved DeepLab V3+ network based coconut CT image segmentation method. Frontiers in Plant Science 14, 1139666.
  • Lin S et al. (2023) Visualization and quantification of coconut using advanced computed tomography postprocessing technology. PLOS ONE 18(2), e0282182.
  • Lin S et al. (2025) The observation of internal structure changes and survival prediction modeling of mature coconut during germination based on computed tomography imaging. Industrial Crops & Products 233, 121396.